A Comparative Study of Three Techniques for Eluting Red Cell Antibodies
OYTIP NATHALANG, M.Sc.*, SASITORN BEJRACHANDRA, M.D.***, SUNEE STHABUNSA W ASDIGARN, B.Sc. **, JARIYA SAIPIN, M.Sc.***, THIP SRIPHAISAL, M.D.*
Affiliation : * Department of Pathology, Pramongkutklao College of Medicine, Bangkok 10400, ** Blood Bank, Ramathibodi Hospital, Bangkok 10400, *** Department of Transfusion Medicine, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
Abstract
Sixty-seven eluates obtained from the heat, ether and acid elution techniques were tested
with the specific red blood cells (RBCs) and were compared according to their reactivities using
the indirect antiglobulin test (IAT). It was found that the ether elution technique was superior in
eluting Rh antibodies except for anti-e while the acid elution technique was superior in eluting
Miltenberger (Mia) antibodies (P < 0.05). The heat elution technique gave the lowest reactivity
among the three techniques.
In conclusion, the reactivities of the eluates obtained from the acid elution technique
were overall comparable to those from the ether elution technique. The acid elution technique is
practical for routine use in most blood banks because it is less time consuming and reduces the
risk of exposing hazardous chemicals.
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