HLA in Southern Thai-Muslims
PIMOL CHIEWSILP,M.D.*, KANCHANA SUJIRACHATO, Ph.D.*, KITTI RATTANASOMBAT, M.D.**, TASANEE MONGKOLSUK, M.Sc.*, SUREEPORN JUNPONG, B.Sc.*, CHAIYOT UDEN, M.Sc.**
Affiliation : * Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, ** Ta Sala Hospital, Nakhon Si Thammarat 80160, Thailand.
Abstract One hundred and two Southern Thai-Muslims (STM) from Nakhon Si Thammarat province were studied for HLA class I and II by SSP ARMS-PCR and PCR-SSO, respectively. The allele frequencies, haplotype frequencies, delta value and linkage disequilibrium between alleles were expressed. The most frequent alleles for HLA-A, HLA-B and HLA-C were A *24(02,03), A* II (0 1 ,02), A *02(0 1,03,05-07,11 ): B * 15(0 1,04-07,12, 19,20), B *07(02-05), B *51 (0 1-05)/B *52 (011,012); and Cw*07(01-03), Cw*04(01,02), Cw*08(01-03), respectively. The HLA class II alleles frequently found were DRB1*1202, DRB1*15021, DRB1*0701; DRB3*0301; DRB5* 0101; DQA1 *0101, DQA1 *0103, DQAl *0601; DQB I *0301, DQB1 *0501, DQBI *0201; and DPB 1 * 1301, DPB 1 *230 1 and DPB 1 *050 1. Two common HLA class I and II haplotypes with significant linkage disequilibrium were A *24 (02,03)-Cw*08 (01-03)-B* 15 (01 ,04-07, 12, 19,20) -DRB1*1202 and A*33 (01,02)-Cw*0302-B*5801-DQB1*0201. The absence of B*27 and DRBI *1401, the presence of A*2301 and high frequency of A*68 were observed in STM. Conclusion : Certain level of genetically distinction among STM, CT and NET existed. However, the genetic diversity of STM was relatively closer to CT than NET.
Keywords :
