DNA Typing of the HLA-A, -B and -C Genes : Possible MHC Class I Haplotypes in the Northeastern-Thais
AMORNRAT ROMPHRUK, M.Sc.*, CHANVIT LEELAYUWAT, Ph.D.*,**, SAHAWAT BARUSRUX,M.Sc.*,**, CHINTANA PUAPAIROJ,M.D.*, ARUNRAT ROMPHRUK, M.Sc.**, YUPA URWUITAROON, M.Sc.*,**
Affiliation : * Blood Transfusion Centre, Faculty of Medicine, Khon Kaen University, ** Department of Clinical Immunology, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
Abstract The phenotype and gene frequencies of HLA class I were studied in the Northeastern Thai population. Blood samples were collected from 100 unrelated healthy northeastern-Thais. HLA-A, -B and -Cw alleles were determined using the polymerase chain reaction- amplification refractory mutation system (PCR-ARMS). 12 HLA-A, 20 HLA-B and 14 HLA-Cw alleles were found. Linkage disequilibrium analysis indicated the existence of 7 HLA-A-B and 19 HLA-B-Cw haplotypes. A*0207-Cw*Ol-B*4601 was the most common possible haplotype in this population. These results provide regional basic information for further studies in anthropology, organ transplantation and MHC disease associations in the northeastern-Thais.
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