The Induction of Cyclooxygenase-2 (COX-2) in Cultured Endothelial Cells Treated with Serum from Preeclampsia is Mediated by lnterleukin-6

PRAVIT AKARASEREENONT, M.D.*, SIRIKUL CHOTEWUTT AKORN, M.Sc. *, KITIRAT TECHATRISAK, M.D.**, ATHIWAT THAWORN,M.S.T.*

Affiliation : * Department of Pharmacology, **Department of Obstetrics and Gynaecology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.

AbstractCOX-2 protein, but not COX-1 protein, was induced in HUVEC from women with a normal pregnancy (nHUVEC) treated with serum from patients with preeclampsia (pSerum). but not with serum from women with a normal pregnancy (nSerum). COX activity in pSerum treated nHUVEC was less than in nSerum treated nHUVEC. Interestingly, the induction of COX-2 pro- tein in nHUVEC treated with pSerum was inhibited by antiiL-6 antibody. The decreased COX activity in nHUVEC treated with pSerum plus antiiL-6 antibody was also reversed in a dose dependent manner. Thus, the induction of COX-2 in pSerum treated nHUVEC was mediated by IL-6. Therefore, the development of selective inhibitors of COX-2 or of IL-6 antagonists may have a potential role in the prevention and treatment of preeclampsia.

Keywords : Preeclampsia, Serum, HUVEC, COX-2

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