Population Genetic Data on Loci LDLR, GYPA, HBGG, D7S8 and GC in the Bangkok Population Compared with Rural Thais from Trat Province
TADA SUEBLINVONG, M.D.*, WILAI ANOMASIRI, Ph.D.*, TANASAK SUEBLINVONG, M.D.*** NANTANA SIRISUP, M.D.**, UNCHALEE KONGSRISOOK, M.Sc. *,
Affiliation : * Department of Biochemistry, ** Department of Forensic Medicine, *** Department of Obstetrics & Gynecology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
Prior to the introduction of any DNA marker as a tool for person identification and pater-
nity test in certain ethnic groups, a population genetic database should be constructed. Using
multiplex primers in single tube polymerase chain amplification, 5 loci of unrelated genes in the PM
Amplitype® kit (Perkin Elmer) were studied in two Thai population groups : 228 DNA samples were
extracted from blood collected at the Borai rural area in Trat province; another 123 DNA samples
were collected at the outpatient clinic, Department of Forensic Medicine, King Chulalongkorn Memo-
rial Hospital, Bangkok. Analysis of alleles and genotypes was performed after reversed dot blot
hybridization of PCR products to allelic sequence specific probes immobilized on the membrane
strip followed by nonradioisotopic detection according to the manufacturer's protocol. Population
genetic statistic parameters including discrimination power (DP), the probability of matching (PM),
power of exclusion for trio (PE trio) and typical paternity index (PI typical) were computed. Both
Thai population groups showed no significant deviation from the Hardy Weinberg Expectation
(HWE). The combined DP of all 5 loci in the PM Amplitype® markers was 0.993636 for rural
Thais and 0.994409 for Thais from Bangkok. The combined PM for rural Thais and those living
in Bangkok was 0.006364 and 0.005591, respectively. The combined PE trio was 0.696825 and
0.698875 in both Thai population groups and the combined PI typical values were <1.0.
In conclusion, person identification using PM Amplitype DNA markers was efficient and
satisfactory within certain limits. Hence, the application of PM Amplitype® DNA markers for
paternity tests should be cautiously considered and applied in combination with other parameters.
Keywords : PM Markers, Population Genetic Database, DNA Markers
