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Objective: To investigate the effect of cowanin on anti-proliferation and apoptosis induction of breast cancer cell, MDA-MB-468.
Material and Method: MTT assay was used for cell proliferation and viability assay. Nuclear morphological changes and mitochondrial membrane potential was determined by Hoechst 33342 staining and JC-1 staining, respectively.
Results: Cowanin showed anti-proliferation and cell viability reduction in MDA-MB-468 cells in a time- and dose-dependent manner with IC50 value of 16.27±0.88 μg/mL. Furthermore, cowanin induced chromatin condensation, apoptotic bodies and mitochondrial membrane dysfunction in MDA-MB-468 cells.
Conclusion: Cowanin showed anti-proliferation and apoptosis induction in MDA-MB-468 cells. These results are basic knowledge for developing the chemotherapeutic agent for breast cancer in the future.
Keywords: Breast cancer cells, Cowanin, Apoptosis, Cells proliferation