Views: 1,382 | Downloads:
23
| Responses: 0
| XML | Respond to this article | Alert & updates | Request permissions | Email to a friend |
Original ArticleOpen Access
Apoptotic Activity in Lymphocytes from Radiation-Treated Cervical Cancer Patients
Sueblinvong T 
,
Pamutha P
,
Pamutha P TADA SUEBLINVONG, MD*,
PISUT PAMUTHA, MSc*
Purpose : The per cent apoptotic activity in lymphocytes from pre-treated cervical cancer
patients was cross-sectionally compared with post-treated patients at 1 month, 3 months, 1 year, and
5 years after completing the standard radiation therapeutic regimen. In addition, the differences in the
per cent apoptotic activities among various stages of cervical cancer were simultaneously analyzed.
Material and Method : Blood samples were collected from five patients in each stage of
cervical cancer before treatment, and at 1 month, 3 months, 1 year, and 5 years after completing the
radiation therapy. The control samples were collected from healthy and aged-match female blood
donors. The lymphocytes were separated and exposed to 0.5 Gy Co irradiation to induce apoptosis.
The apoptotic cells were detected by
in situ
terminal deoxynucleotidyl transferase (TdT assay) and
counted under a fluorescent microscope. Both the apoptotic index and per cent apoptotic activity were
calculated.
Results : The per cent apoptotic activities in lymphocytes from all pre-treated patients with
stage II and III cervical cancer were significantly lower than the controls (p
=
0.001). The apoptotic
activity in normal control, however, was not significantly different from the pre-treated stage I cervical
cancer group. Following radiation therapy, the apoptotic activities at 1 month, 3 month and 1 year
were increased in all stages. The per cent apoptotic activity, in all stages of cervical cancer at 5 years
after treatment, was statistically higher than that of the pre-treated groups.
Conclusion : There was a decrease of per cent apoptotic activity in lymphocytes from all
pre-treated cervical cancer patients in the present study, the change of which was reversed to normal
after treatment in non-recurrent cases.
Key word : Apoptosis, Lymphocyte Apoptotic Activity, Cervical Cancer, Radiation Therapy for
Cervical Cancer
PISUT PAMUTHA, MSc*
Purpose : The per cent apoptotic activity in lymphocytes from pre-treated cervical cancer
patients was cross-sectionally compared with post-treated patients at 1 month, 3 months, 1 year, and
5 years after completing the standard radiation therapeutic regimen. In addition, the differences in the
per cent apoptotic activities among various stages of cervical cancer were simultaneously analyzed.
Material and Method : Blood samples were collected from five patients in each stage of
cervical cancer before treatment, and at 1 month, 3 months, 1 year, and 5 years after completing the
radiation therapy. The control samples were collected from healthy and aged-match female blood
donors. The lymphocytes were separated and exposed to 0.5 Gy Co irradiation to induce apoptosis.
The apoptotic cells were detected by
in situ
terminal deoxynucleotidyl transferase (TdT assay) and
counted under a fluorescent microscope. Both the apoptotic index and per cent apoptotic activity were
calculated.
Results : The per cent apoptotic activities in lymphocytes from all pre-treated patients with
stage II and III cervical cancer were significantly lower than the controls (p
=
0.001). The apoptotic
activity in normal control, however, was not significantly different from the pre-treated stage I cervical
cancer group. Following radiation therapy, the apoptotic activities at 1 month, 3 month and 1 year
were increased in all stages. The per cent apoptotic activity, in all stages of cervical cancer at 5 years
after treatment, was statistically higher than that of the pre-treated groups.
Conclusion : There was a decrease of per cent apoptotic activity in lymphocytes from all
pre-treated cervical cancer patients in the present study, the change of which was reversed to normal
after treatment in non-recurrent cases.
Key word : Apoptosis, Lymphocyte Apoptotic Activity, Cervical Cancer, Radiation Therapy for
Cervical Cancer
Download:
PDF