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Original ArticleOpen Access
Normal Plasma Free Amino Acid Levels in Thai Children
Svasti J 
,
Srisomsap C ,
Wasant P ,
Pangkanon S ,
Tiensuwan M ,
Boonpuan K ,
Sawangareetrakul P ,
Liammongkolkul S
,
Srisomsap C ,
Wasant P ,
Pangkanon S ,
Tiensuwan M ,
Boonpuan K ,
Sawangareetrakul P ,
Liammongkolkul S Analysis of plasma free amino acid levels is important for diagnosis of inborn errors of
metabolism. Traditionally, this is performed using commercially available dedicated amino acid
analyzers, but few such instruments are available in Thailand, and many are not used in routine
operations. Here, the authors describe the analysis of plasma free amino acid levels in 57 normal
children by reverse-phase HPLC and pre-column derivatization with phenylisothiocyanate. Plasma
free amino levels are reported as mean
เธ‘
SD and 95 per cent confidence interval of mean for
each of 5 age groups: 0-6 months; 6-12 months; 1-3 years; 3-6 years; 6-12 years. Mean amino
acid levels were generally similar in all age groups (p2!0.01), except that hydroxyproline tended to
be higher in the 0-6 months age group compared to other age groups (p
metabolism. Traditionally, this is performed using commercially available dedicated amino acid
analyzers, but few such instruments are available in Thailand, and many are not used in routine
operations. Here, the authors describe the analysis of plasma free amino acid levels in 57 normal
children by reverse-phase HPLC and pre-column derivatization with phenylisothiocyanate. Plasma
free amino levels are reported as mean
เธ‘
SD and 95 per cent confidence interval of mean for
each of 5 age groups: 0-6 months; 6-12 months; 1-3 years; 3-6 years; 6-12 years. Mean amino
acid levels were generally similar in all age groups (p2!0.01), except that hydroxyproline tended to
be higher in the 0-6 months age group compared to other age groups (p
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