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Original ArticleOpen Access
Identification of Sex Chromosome by Fluorescence in situ Hybridization
Sarasoplwna S 
,
Promsawat K ,
Rerkamnuaychok B
,
Promsawat K ,
Rerkamnuaychok B Fluorescence
in situ
hybridization (FISH) is the complicated and very effective technique
to determine the origin of chromosome material that cannot be identified by conventional banding
techniques. Also determining the hidden sex chromosome and the percentage of mosaicism. Five
peripheral blood and. one cord blood sample were used to perform centromeric X and Y chromo-
some-specific DNA probe to determine the sex chromosome. Comparing the percentage of mosai-
cism between conventional cytogenetic technique and FISH technique, we found a different ratio in
mosaicism. That is because the molecular cytogenetic study was the evaluation of chromosome
identification in both dividing (metaphase) and non-dividing cells (interphase nuclei).
Key word : FISH, Sex Chromosome
in situ
hybridization (FISH) is the complicated and very effective technique
to determine the origin of chromosome material that cannot be identified by conventional banding
techniques. Also determining the hidden sex chromosome and the percentage of mosaicism. Five
peripheral blood and. one cord blood sample were used to perform centromeric X and Y chromo-
some-specific DNA probe to determine the sex chromosome. Comparing the percentage of mosai-
cism between conventional cytogenetic technique and FISH technique, we found a different ratio in
mosaicism. That is because the molecular cytogenetic study was the evaluation of chromosome
identification in both dividing (metaphase) and non-dividing cells (interphase nuclei).
Key word : FISH, Sex Chromosome
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