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Original ArticleOpen Access
Investigation of Sex Chromosome Abnormalities in Teratozoospermia of Infertile Men using Fluorescence in Situ Hybridization Anek Aribarg, F.R.C.O.G.,
Ngeamvijawat J 
,
Chanprasit Y
,
Chanprasit Y Fluorescence
in situ
hybridization is a fast and efficient method of investigating chro-
mosomal abnormalities in human
s~fi!latp_zQ_a.
In this study, we have established the frequency
of sex chromosome disomy (XX, XY and YY) in teratozoospermia (98-1
OOlk
abnormal mor-
phology) of infertile men compared with normospermic men using double-probe FISH pro-
cedures. A total of 40000 sperms were scored in each group of semen for chromosome X and Y
with overall hybridization efficiency of 97.00 per cent and 98.02 per cent in infertile men and
normal men, respectively. It was found that the frequency of disomy XX, XY and YY was sig-
nificantly higher in infertile men compared with normal men (P
in situ
hybridization is a fast and efficient method of investigating chro-
mosomal abnormalities in human
s~fi!latp_zQ_a.
In this study, we have established the frequency
of sex chromosome disomy (XX, XY and YY) in teratozoospermia (98-1
OOlk
abnormal mor-
phology) of infertile men compared with normospermic men using double-probe FISH pro-
cedures. A total of 40000 sperms were scored in each group of semen for chromosome X and Y
with overall hybridization efficiency of 97.00 per cent and 98.02 per cent in infertile men and
normal men, respectively. It was found that the frequency of disomy XX, XY and YY was sig-
nificantly higher in infertile men compared with normal men (P
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