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Original ArticleOpen Access
DNA Extraction and Amplification of 10-day, Room-Temperature Blood Samples
Sasanakul W 
,
Chuansumrit A ,
Rurgkhum S ,
Udomsubpayakul U ,
Hathirat P
,
Chuansumrit A ,
Rurgkhum S ,
Udomsubpayakul U ,
Hathirat P DNA was serially studied in 20 samples of buffy coat stored at room temperature. Each
sample was divided into 5 equal volumes, namely D0, D3, D5, D7 and D10. DNA extraction was
performed on days 0, 3, 5, 7 and 10 after blood collection. The mean ratio of OD260/0D280 of
the DNA obtained from Do to D10 ranged from 1.77 to 1.79, and the mean amounts of the DNA
obtained from Do to D10 ranged from 602 to 740 ng/ul. There were no significant differences in
the mean ratio and amounts of DNA obtained among these samples (p >0.05). Subsequently,
amplification was successfully performed from this template DNA to yield products of 1.4 kb
and 142 bp at the sites associated with B globin and factor VIII genes, respectively. These findings
suggest the possibility of sending blood samples for DNA analysis by mail, or no ice is required
during transportation.
Key word : DNA Extraction, Buffy Coat
sample was divided into 5 equal volumes, namely D0, D3, D5, D7 and D10. DNA extraction was
performed on days 0, 3, 5, 7 and 10 after blood collection. The mean ratio of OD260/0D280 of
the DNA obtained from Do to D10 ranged from 1.77 to 1.79, and the mean amounts of the DNA
obtained from Do to D10 ranged from 602 to 740 ng/ul. There were no significant differences in
the mean ratio and amounts of DNA obtained among these samples (p >0.05). Subsequently,
amplification was successfully performed from this template DNA to yield products of 1.4 kb
and 142 bp at the sites associated with B globin and factor VIII genes, respectively. These findings
suggest the possibility of sending blood samples for DNA analysis by mail, or no ice is required
during transportation.
Key word : DNA Extraction, Buffy Coat
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