Views: 1,413 | Downloads:
17
| Responses: 0
| XML | Respond to this article | Alert & updates | Request permissions | Email to a friend |
Original ArticleOpen Access
Cryopreservation of Human Spermatozoa : Comparison of Two Cryoprotectants and Two Freezing Methods
Julavijitphong S 
,
Kunathikom S ,
Suksompong S ,
Mekemahan O ,
Dangratana J
,
Kunathikom S ,
Suksompong S ,
Mekemahan O ,
Dangratana J A prospective experimental study was performed to compare sperm cryosurvival rate
between two kinds of cryoprotective media: citrate-egg yolk-glycerol (CEG) and TEST-citrateegg
yolk-glycerol (TEST-EYG), and between two freezing methods: noncontrolled rate (NCR) freezing
and computer controlled rate (CR) freezing. One hundred semen samples were included in the
study. Each sample was divided into two parts, which were prepared as two CEG and two TESTEYG
mixture straws, then one straw from each mixture was frozen by the NCR-freezing method
and the complementary straw was frozen by the CR-freezing method. After one week of cryostorage,
the straws were thawed and the post-thaw sperm survival was assessed. As the results, the
post-thaw sperm survival rate of the CEG media group was slightly higher than the TEST-EYG
group in both the NCR-freezing method (51.2% vs 47.0%, p < 0.05) and the CR-freezing method
(48.2% vs 43.4%, p < 0.05); and similarly; the NCR-freezing group yielded a slightly higher postthaw
sperm survival rate than the CR-freezing group in both the CEG media used (51.2% vs 48.2%,
p < 0.05) and the TEST-EYG media used (47.0% vs 43.4%, p < 0.05). However, these did not have
clinical significance because the differences were too small. In conclusion, the use of either CEG
or TEST-EYG media, and either the NCR or CR-freezing method did not have clinical differences
on the post-thaw sperm survival.
between two kinds of cryoprotective media: citrate-egg yolk-glycerol (CEG) and TEST-citrateegg
yolk-glycerol (TEST-EYG), and between two freezing methods: noncontrolled rate (NCR) freezing
and computer controlled rate (CR) freezing. One hundred semen samples were included in the
study. Each sample was divided into two parts, which were prepared as two CEG and two TESTEYG
mixture straws, then one straw from each mixture was frozen by the NCR-freezing method
and the complementary straw was frozen by the CR-freezing method. After one week of cryostorage,
the straws were thawed and the post-thaw sperm survival was assessed. As the results, the
post-thaw sperm survival rate of the CEG media group was slightly higher than the TEST-EYG
group in both the NCR-freezing method (51.2% vs 47.0%, p < 0.05) and the CR-freezing method
(48.2% vs 43.4%, p < 0.05); and similarly; the NCR-freezing group yielded a slightly higher postthaw
sperm survival rate than the CR-freezing group in both the CEG media used (51.2% vs 48.2%,
p < 0.05) and the TEST-EYG media used (47.0% vs 43.4%, p < 0.05). However, these did not have
clinical significance because the differences were too small. In conclusion, the use of either CEG
or TEST-EYG media, and either the NCR or CR-freezing method did not have clinical differences
on the post-thaw sperm survival.
Download:
PDF