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Original ArticleOpen Access
Mucin Production in Prostatic Adenocarcinoma: A Retrospective Study of 190 Radical Prostatectomy and/ or Core Biopsy Specimens in Department of Pathology, Siriraj Hospital, Mahidol University, Thailand
Noiwan S 
,
Rattanarapee S
,
Rattanarapee S Background: Diagnoses of prostatic adenocarcinoma are made based on a constellation of architectural, cytological, andancillary findings, which include intraluminal mucin secretion. Several studies have shown that luminal acid mucin isfrequently present in prostatic adenocarcinoma. Immunohistochemical stain (immunostaining) becomes extremely helpful inthe diagnosis of prostatic adenocarcinoma, especially in doubtful cases. However, presently, the technique is not available inmost hospitals, particularly in the regional or provincial areas. Thus, luminal acid mucin and its detection by histochemicalstains may be one of the useful tools in diagnosing prostatic adenocarcinoma.
Objective: To determine the percentage of mucin production in prostatic adenocarcinoma from 190 radical prostatectomyand/or core biopsy specimens in the Departmeant of Pathology, Siriraj Hospital, Mahidol University, to compare thepercentage of positivity among three different special histochemical stains (mucicarmine, Alcian blue at pH 2.5, and colloidaliron), and to determine the PSA-expression among the prostatic adenocarcinoma that produces mucin by immunohistochemicaltechnique.
Material and Method: The present study is a retrospective study of 190 cases that were diagnosed as prostatic adenocarcinoma(with any Gleason’s microscopic pattern) from radical prostatectomy and/or core biopsy specimens in Department ofPathology, Siriraj Hospital, Mahidol University between January 2006 and May 2007. All cases were retrieved from acomputer filing system of the Department. All H&E slides that contained diagnostic materials were reviewed and stained withall three different special histochemical stains. Positive cases were subsequently studied to find PSA expression by means ofimmunohistochemical study. Cases with microscopic variants of prostatic adenocarcinoma and metastasis were excluded.
Results: One hundred and seventy seven cases (93%) of these prostatic adenocarcinomas were positive for at least one ofthree different special histochemical stains for acid mucin. Among these, 167 cases (88%) were positive for mucicarmine, 157(83%) cases were positive for Alcian blue at pH 2.5, and 173 cases (91%) were positive for colloidal iron. All 177 cases werepositive for PSA immunohistochemical stain.
Conclusion: Intraluminal acid mucin and its detection by special histochemical stains is one of the useful methods (with a highpercentage of positivity) in diagnosing prostatic adenocarcinoma in association with other architectural and cytologicalcriteria. Mucicarmine and colloidal iron stains appear to be more useful than Alcian blue at pH 2.5 stain according to theirpositivity percentage. However, in pathologic practice, mucicarmine stain is more commonly used than colloid iron stain dueto the difficulty in preparation and interpretation of the latter. There is no correlation between mucin production and PSAexpression in prostatic adenocarcinoma but PSA immunohistochemical stain is still useful to confirm the prostatic origin ofpoorly differentiated carcinomas or metastatic lesions.
Keywords: Prostatic adenocarcinoma, Intraluminal acid mucin, Radical prostatectomy, Core biopsy, Mucicarmine histochemicalstain, Alcian blue at pH 2.5 histochemical stain, Colloidal iron histochemical stain, PSA immunohistochemical stain
Objective: To determine the percentage of mucin production in prostatic adenocarcinoma from 190 radical prostatectomyand/or core biopsy specimens in the Departmeant of Pathology, Siriraj Hospital, Mahidol University, to compare thepercentage of positivity among three different special histochemical stains (mucicarmine, Alcian blue at pH 2.5, and colloidaliron), and to determine the PSA-expression among the prostatic adenocarcinoma that produces mucin by immunohistochemicaltechnique.
Material and Method: The present study is a retrospective study of 190 cases that were diagnosed as prostatic adenocarcinoma(with any Gleason’s microscopic pattern) from radical prostatectomy and/or core biopsy specimens in Department ofPathology, Siriraj Hospital, Mahidol University between January 2006 and May 2007. All cases were retrieved from acomputer filing system of the Department. All H&E slides that contained diagnostic materials were reviewed and stained withall three different special histochemical stains. Positive cases were subsequently studied to find PSA expression by means ofimmunohistochemical study. Cases with microscopic variants of prostatic adenocarcinoma and metastasis were excluded.
Results: One hundred and seventy seven cases (93%) of these prostatic adenocarcinomas were positive for at least one ofthree different special histochemical stains for acid mucin. Among these, 167 cases (88%) were positive for mucicarmine, 157(83%) cases were positive for Alcian blue at pH 2.5, and 173 cases (91%) were positive for colloidal iron. All 177 cases werepositive for PSA immunohistochemical stain.
Conclusion: Intraluminal acid mucin and its detection by special histochemical stains is one of the useful methods (with a highpercentage of positivity) in diagnosing prostatic adenocarcinoma in association with other architectural and cytologicalcriteria. Mucicarmine and colloidal iron stains appear to be more useful than Alcian blue at pH 2.5 stain according to theirpositivity percentage. However, in pathologic practice, mucicarmine stain is more commonly used than colloid iron stain dueto the difficulty in preparation and interpretation of the latter. There is no correlation between mucin production and PSAexpression in prostatic adenocarcinoma but PSA immunohistochemical stain is still useful to confirm the prostatic origin ofpoorly differentiated carcinomas or metastatic lesions.
Keywords: Prostatic adenocarcinoma, Intraluminal acid mucin, Radical prostatectomy, Core biopsy, Mucicarmine histochemicalstain, Alcian blue at pH 2.5 histochemical stain, Colloidal iron histochemical stain, PSA immunohistochemical stain
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