Views: 1,870 | Downloads:
187
| Responses: 0
| XML | Respond to this article | Alert & updates | Request permissions | Email to a friend |
Original ArticleOpen Access
Cytotoxic Effect and Its Mechanism of Dioscorealide B from Dioscorea membranacea against Breast Cancer Cells
Saekoo J 
,
Dechsukum C ,
Graidist P ,
Itharat A
,
Dechsukum C ,
Graidist P ,
Itharat A Background: Dioscorealide B is an active compound from the rhizome of Dioscorea membranacea Pierre which locallyknown as “Hua-Khao-Yen”. This medicinal plant has long been used in the anticancer prescription of Thai traditionalmedicine..
Objective: To examine the cytotoxic effect and mechanism of Dioscorealide B in human breast carcinoma cells.
Material and Method: Dioscorealide B was isolated from the rhizome of Hua KhaoYen (Dioscorea membranacea).Thecytotoxicity of Dioscorealide B was evaluated in two human breast cancer cell lines, MCF-7 and MDA-MB 468 bySulphorhodamine B (SRB) assay. RT-PCR and Caspase-Glo® assay were used to further elucidate its cytotoxic mechanism.
Results: Dioscorealide B showed cytotoxic effect on MCF-7 (IC50=2.76 μM) and MDA-MB 468 (IC50=9.93 μM). The mRNAlevel for p53, p21 and Bax were increased while Bcl-2 was decreased after the treatment. MCF-7 treated with DioscorealideB showed the induction of apoptosis via the activation of caspase-9 and -7
Conclusion: The results suggested that the mechanisms of Dioscorealide B might be involved in p53 and the intrinsicapoptotic pathway
Keywords: Dioscorea membranace, Dioscorealide B, SRB assay, Apoptosis, MCF-7
Objective: To examine the cytotoxic effect and mechanism of Dioscorealide B in human breast carcinoma cells.
Material and Method: Dioscorealide B was isolated from the rhizome of Hua KhaoYen (Dioscorea membranacea).Thecytotoxicity of Dioscorealide B was evaluated in two human breast cancer cell lines, MCF-7 and MDA-MB 468 bySulphorhodamine B (SRB) assay. RT-PCR and Caspase-Glo® assay were used to further elucidate its cytotoxic mechanism.
Results: Dioscorealide B showed cytotoxic effect on MCF-7 (IC50=2.76 μM) and MDA-MB 468 (IC50=9.93 μM). The mRNAlevel for p53, p21 and Bax were increased while Bcl-2 was decreased after the treatment. MCF-7 treated with DioscorealideB showed the induction of apoptosis via the activation of caspase-9 and -7
Conclusion: The results suggested that the mechanisms of Dioscorealide B might be involved in p53 and the intrinsicapoptotic pathway
Keywords: Dioscorea membranace, Dioscorealide B, SRB assay, Apoptosis, MCF-7
Download:
PDF