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Original ArticleOpen Access
Effect of Dialyzer Membranes on Beta-2 Microglobulin Production in Thai Hemodialysis Patients
Domrongkitchaiporn S 
,
Chuncharunee S ,
Archararit N ,
Atamasirikul K ,
Vanichakarn S
,
Chuncharunee S ,
Archararit N ,
Atamasirikul K ,
Vanichakarn S Responses to different types of dialyzer membranes in an Asian population may differ
from those of a Caucasian population. Comparative studies on the effects of different dialyzer
membranes on beta-2 microglobulin production are also limited. Therefore, we conducted this
study to determine the effects of different dialyzer membranes on in vitro mononuclear cell production
of beta-2 microglobulin in 9 Thai hemodialysis patients. Each patient was dialysed with
4 different types of dialyzer, including cuprophane (CUP), cellulose diacetate (CD), polysulphone
(PS), and polyacrylonitrile membrane (PAN), each for a 1-month period in a randomized sequence.
Mononuclear cell culture was done by taking an immediate post-dialysis blood sample at the end
of the 1-month period. Beta-2 microglobulin production from cell culture was determined 24 hours
later. Mononuclear cell culture and determination of beta-2 microglobulin production from the
culture were also done in 10 normal controls and 10 predialysis ESRD patients. The beta-2 microglobulin
productions (jlg/L) were shown as follows;
Control CUP CD PS PAN
Normal controls 169±18* - - - -
Predialysis ESRD 162±29* - - - -
Hemodialysis pateints - 268±47 198±4 175±40* 173±20*
(* p < 0.05 compared to cuprophane membrane)
Conclusion: polysulphone and polyacrylonitrile membrane induced significantly less beta-2 microglobulin
production compared to cuprophane and slightly less compared to cellulose diacetate
membrane.
from those of a Caucasian population. Comparative studies on the effects of different dialyzer
membranes on beta-2 microglobulin production are also limited. Therefore, we conducted this
study to determine the effects of different dialyzer membranes on in vitro mononuclear cell production
of beta-2 microglobulin in 9 Thai hemodialysis patients. Each patient was dialysed with
4 different types of dialyzer, including cuprophane (CUP), cellulose diacetate (CD), polysulphone
(PS), and polyacrylonitrile membrane (PAN), each for a 1-month period in a randomized sequence.
Mononuclear cell culture was done by taking an immediate post-dialysis blood sample at the end
of the 1-month period. Beta-2 microglobulin production from cell culture was determined 24 hours
later. Mononuclear cell culture and determination of beta-2 microglobulin production from the
culture were also done in 10 normal controls and 10 predialysis ESRD patients. The beta-2 microglobulin
productions (jlg/L) were shown as follows;
Control CUP CD PS PAN
Normal controls 169±18* - - - -
Predialysis ESRD 162±29* - - - -
Hemodialysis pateints - 268±47 198±4 175±40* 173±20*
(* p < 0.05 compared to cuprophane membrane)
Conclusion: polysulphone and polyacrylonitrile membrane induced significantly less beta-2 microglobulin
production compared to cuprophane and slightly less compared to cellulose diacetate
membrane.
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